Targeted bisulfite sequencing by solution hybrid selection and massively parallel sequencing
نویسندگان
چکیده
منابع مشابه
Targeted bisulfite sequencing by solution hybrid selection and massively parallel sequencing
We applied a solution hybrid selection approach to the enrichment of CpG islands (CGIs) and promoter sequences from the human genome for targeted high-throughput bisulfite sequencing. A single lane of Illumina sequences allowed accurate and quantitative analysis of ~1 million CpGs in more than 21,408 CGIs and more than 15,946 transcriptional regulatory regions. Of the CpGs analyzed, 77-84% fell...
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DNA methylation is one of the most important epigenetic alterations involved in the control of gene expression. Bisulfite sequencing of genomic DNA is currently the only method to study DNA methylation patterns at single-nucleotide resolution. Hence, next-generation sequencing of bisulfite-converted DNA is the method of choice to investigate DNA methylation profiles at the genome-wide scale. Ne...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2011
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkr598